mouse anti-human bmp4  (R&D Systems)

Journal: Respiratory research

Article Title: Levosimendan mediates the BMP/Smad axis through upregulation of circUSP34-targeted miR-1298 to alleviate pulmonary hypertension.

doi: 10.1186/s12931-024-02945-5

Figure Lengend Snippet: Fig. 5 Lev upregulates circUSP34-targeted miR-1298-mediated BMP/Smad axis to inhibit cell proliferation, migration and promote apoptosis in hypoxia- treated PASMCs. (A) The expression of BMPR2 in PASMCs was measured by WB. (B) The protein expression levels of BMPR2 in PASMCs under different treatment conditions were detected by WB. (C) The cell proliferation ability of PASMCs under different treatment conditions was examined by CCK-8. (D) The expression of Ki-67 in PASMCs was measured by IF. (E) Cell migration ability of PASMCs was tested by cell scratch assay. (F) Apoptosis of PASMCs was determined by flow cytometry. (G) WB was performed to test the protein expression levels of BMP4, p-Smad1/5/8, Id1 in PASMCs under different treat ment conditions. Data are displayed for three individual experiments with mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001

Article Snippet: After blocking with 5% skim milk for 1 h, the membranes were kept at 4 °C and incubated overnight with the following primary antibodies: BMPR2 (1:1000, Abcam, ab130206), BMP4 (1:1000, Cell Signaling Technology, 4680), Smad1/5/8 (1:1000, Abcam, ab13723), p-Smad1/5/8 (1:1000, Sigma-Aldrich, AB3848-I), Id1 (1:1000, Abcam, ab168256), β-actin (1:2000, Cell Signaling Technology, 4967).

Techniques: Migration, Expressing, CCK-8 Assay, Wound Healing Assay, Flow Cytometry

Journal: Respiratory research

Article Title: Levosimendan mediates the BMP/Smad axis through upregulation of circUSP34-targeted miR-1298 to alleviate pulmonary hypertension.

doi: 10.1186/s12931-024-02945-5

Figure Lengend Snippet: Fig. 6 Lev upregulates circUSP34-targeted miR-1298-mediated BMP/Smad axis to alleviate hypoxia combined with SU5416-induced PH. (A) Detection of RVSP in PH rat. (B) The mPAP levels were measured in PH rat. (C) Detection of RVI in PH rat. (D) Detection of LVSP in PH rat. (E) Examination of vascular wall changes in PH rat by HE staining. (F) The collagen deposition phenomenon in the lung interstitial tissue in PH rat was detected by Masson staining. (G) Detection of Ki-67 expression in pulmonary artery tissues in PH rat by IHC. (H) Apoptosis in the pulmonary artery tissue in PH rat was measured by TUNEL. (I) Levels of circUSP34 and miR-1298 were probed by qRT-PCR in PH rat. (J) WB was performed to measure the expression levels of BMPR2, BMP4, p-Smad1/5/8, and Id1 proteins in PH rat under different treatment conditions. n = 6, *P < 0.05, **P < 0.01, ***P < 0.001

Article Snippet: After blocking with 5% skim milk for 1 h, the membranes were kept at 4 °C and incubated overnight with the following primary antibodies: BMPR2 (1:1000, Abcam, ab130206), BMP4 (1:1000, Cell Signaling Technology, 4680), Smad1/5/8 (1:1000, Abcam, ab13723), p-Smad1/5/8 (1:1000, Sigma-Aldrich, AB3848-I), Id1 (1:1000, Abcam, ab168256), β-actin (1:2000, Cell Signaling Technology, 4967).

Techniques: Staining, Expressing, TUNEL Assay, Quantitative RT-PCR